韩国成人av免费一区二区,一进一出流出白浆视频,日本一本二本不卡视频,中文字幕日韩精品人妻,欧美日韩亚洲中文另类,澳门蜜桃av成人av,久草福利在线视频资源站,国产十日韩十另类十视频新区,伊人五月天婷婷成人

【文獻標題】Effects of rearing systems on reproductive hormones secretion and their receptors gene expression in Xianju chickens under summer conditions

【作者】Z. Z. Yin，X. Y. Dong，H. Y. Cao，et.al

【作者單位】浙江大學動物科學學院（Animal Science College, Zhejiang University）

【文獻中引用產品】

放射免疫分析試劑盒

【關鍵詞】Xianju chicken, reproductive hormone, gene expression, rearing system, summer conditions

【DOI】dx.doi.org/10.3382/ps/pey194

【影響因子(IF)】3.5

【出版期刊】《Poultry Science》

【產品原文引用】

Blood sampling and analysis

At the end of wk 29, 12 h after feed withdrawal, 2 birds were randomly selected from each replicate and blood samples were collected from the axillary vein.Serum was isolated by centrifugation of blood at 3000×g for 10 min, and was stored in 1.5-mL Eppendorf tubes at −70?C until analyses. Serum FSH, P4, LH, and PRL levels were analyzed using RIA kits (Hengyuan Biological Technology Co., Ltd, Shanghai, China) by radio immunoassay, according to the recommendations of the manufacturer. For measurement of FSH concentration, the assay sensitivity, measuring range, intraand interassay CVs were 0.4 mIU/mL, 1.5 mIU/mL to 100mIU/mL, <10% and <15%, respectively. For measurement of P4 concentration, the assay sensitivity was 0.2 ng/mL, measuring range was 0.5 to 80 ng/mL, and the intra- and interassay CVs were <10%. For measurement of LH concentration, the assay sensitivity, measuring range, intra-and interassay CVs were 0.02, 0.5 to 20 ng/mL, <10% and <15%, respectively. For measurement of PRL concentration, the assay sensitivity was 0.5 ng/mL, measuring range was 5.0 to 100 ng/mL,and the intra- and interassay CVs were <10%. All the samples were measured in one single assay.

完整版PDF文獻請咨詢在線客服或者聯系我司業務員！

更多公司福利請關注“恒遠生物”VX公眾號！