韩国成人av免费一区二区,一进一出流出白浆视频,日本一本二本不卡视频,中文字幕日韩精品人妻,欧美日韩亚洲中文另类,澳门蜜桃av成人av,久草福利在线视频资源站,国产十日韩十另类十视频新区,伊人五月天婷婷成人

【文獻(xiàn)標(biāo)題】Programming changes in GLUT1 mediated the accumulation of AGEs and matrix degradation in the articular cartilage of female adult rats after prenatal caffeine exposure

【作者】Qing-xian Li，Lin-long Wang，et.al

【作者單位】武漢大學(xué)中南醫(yī)院（Zhongnan Hospital of Wuhan University）

【文獻(xiàn)中引用產(chǎn)品】

試劑-藏紅o試劑(CAS. No. 477-73-6)

試劑-固綠FCF試劑(CAS. No. 2353-45-9)

【關(guān)鍵詞】Prenatal caffeine exposure，Glucocorticoid，Glucose transporter 1，Glucose metabolism，Articular cartilage，Cartilage development

【DOI】doi.org/10.1016/j.phrs.2019.104555

【影響因子(IF)】9.90

【出版期刊】《Pharmacological Research》

【產(chǎn)品原文引用】

Caffeine (CAS #58-08-2, > 99% purity) was purchased from SigmaAldrich Co., Ltd. (St. Louis, MO, USA). Isoflurane was purchased from Baxter Healthcare Co. (Deerfield, IL, USA). Safranin O (CAS No. 477-73-6) and Fast Green (CAS No. 2353-45-9) were obtained from Hengyuan Biotech (Shanghai, China). A TRIzol reagent (SKU: R6830-02) was purchased from Omega Bio-Tek (Doraville, GA, USA). Reverse transcription and quantitative PCR kits were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Oligonucleotide primers for the gene used for analysis were synthesized by Sangon Biotech Co., Ltd.(Shanghai, China). RIPA lysis buffer and a BCA Protein Assay kit were purchased from Beyotime Institute of Biotechnology (Haimen, China).Western Bright ECL HRP substrate was purchased from Advansta (Menlo Park, CA, USA). Fetal bovine serums were supplied by Gibco (St.Louis, MO, USA). Recombinant Murine IGF1 (250-19) was purchased from PeproTech, Inc. (Rocky Hill, NJ, USA). Advanced glycation end products (AGE-BSA, 2221-BSA) and BSA (2221-10) were purchased from BioVision (Milpitas, CA, USA). Low molecular weight heparin (LMWH) was purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO,USA). Collagenase II was obtained from Invitrogen (Carlsbad, CA, USA).Polyclonal antibody information is as follows: glyceraldehyde 3-phosphatedehydrogenase (GAPDH) (No. AC033), anti-β-actin (No.AB_2768234), α1 chain of type II collagen gene (COL2A1) (No.ABP51023), aggrecan (ACAN) (No. bs-1223R), GLUT1 (No. 21829-1-AP), AGEs (No. bs-1158R), ADAM metallopeptidase with thrombospondin type 1 motif 5 (ADAMTS-5) (No. ab41037), matrix metallopeptidase 13 (MMP13) (No. ab39012), IGF1 (No. sc-9013), and acetylhistone 3 lysine 9 (H3K9) (No. A7255). Secondary antibody information is as follows: Cy3 conjugated goat anti-rabbit IgG (H + L) (No.B100801) and Cy3 conjugated goat anti-rabbit IgG (H + L) (No. AS-1111). The other chemicals and agents were analytical grade.

完整版PDF文獻(xiàn)請(qǐng)咨詢?cè)诰€客服或者聯(lián)系我司業(yè)務(wù)員！

更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”vx公眾號(hào)！